Switzerland's attorney general has opened the case against the Qatari businessman over concerns that he attempted to bribe ex-FIFA secretary general Jerome Valcke for the rights to show the World Cup on BeIn Sports - the broadcasting company at which that Al-Khelaifi is chief executive.
Swiss prosecutors said the action was carried out with Eurojust, a European Union agency for cooperation in criminal matters.
His involvement is a direct link to Qatar, host of the 2022 World Cup, in investigations led by the United States and Swiss into football corruption.
Nasser Al-Khelaifi, the Qatari president of Paris Saint-Germain, is facing criminal proceedings in Switzerland in connection with the award of media rights for certain countries at the FIFA World Cup.
The businessman is the head of Qatari television network beIN Sports, which was awarded tournament media rights by Federation Internationale de Football Association.
The criminal proceeding was opened on March 20, but announced only on Thursday, the Swiss federal office said.
Al-Khelaifi's profile has risen in recent weeks as PSG pursued and sealed a world record transfer of Brazil star Neymar from Barcelona for £198m ($260 million). BeIN has also acquired a broad portfolio of rights including from European soccer body UEFA for the Champions League and national team matches.
In March a year ago, the OAG said Valcke was suspected of criminal mismanagement and other offences.
The investigation was opened in March and is separate from a probe Switzerland launched against Valcke in March of 2016 over alleged criminal mismanagement during his tenure as deputy to FIFA's disgraced former president Sepp Blatter. The DoJ has indicted or secured guilty pleas from more than 40 people.