"Winfrey's projects will be released as part of a lineup of original content from Apple", the company said.
The deal has been described as a "multiyear" one, although the exact number of years is unknown.
Apple has deals with other stars including director Steven Spielberg and actresses Jennifer Aniston and Reese Witherspoon.
Winfrey founded and heads the OWN channel as chair and CEO. She is precluded from appearing on-camera for other cable networks. Sources tell Variety that Apple's deal with Winfrey does not conflict with the Discovery agreement.
Winfrey, considered by some to be the most influential woman in the world, is perhaps best known for hosting The Oprah Winfrey Show which aired nationally from 1986 through 2011.
Winfrey also runs O, The Oprah Magazine and published the New York Times best-selling cookbook "Food, Health and Happiness" past year.
She's also a notable philanthropist, having contributed more than $100 million to provide education to academically gifted girls from disadvantaged backgrounds, and is continually discussed as a potential presidential candidate, though she said that's not for her.
Even before the Oprah deal, Apple has a robust slate of originals prepared to launch. Apple and Amazon have now been seeking out other top talents by offering "more than typical overall deals used to include".
This latest content partnership comes on the heels of writers and producers like Shonda Rhimes and Ryan Murphy landing deals at Netflix.