His final wager was carefully chosen so that he could end up with exactly $110,914, a number he has admitted references his daughter's birthday of November 9, 2014.
Holzhauer's wife, Melissa Sassin, is also an accomplished game show participant, having won $28,800 on Who Wants To Be A Millionaire back in 2012.
Tuesday's win was the fourth straight for Holzhauer and brought his winnings total to more than $244,000. Wednesday's episode will pit him against two new challengers eager to prevent him from scoring a fifth consecutive win.
While plenty of viewers were no doubt scratching their heads at that one, Holzhauer correctly wrote down "WHAT IS QUANTUM LEAP".
"I said all along that I wanted to break Roger Craig's one-game record, and I did it", Holzhauer said, via ABC 11.
Furthermore, he appeared on the show 500 Questions in 2015, heavy.com reported.
James Holtzhauer, 34, broke a record on the game show Thursday when he racked up $110,914, the most any contestant has ever won in a single game. The trailer ends with host Alex Trebek asking, "Is it too soon to begin making comparisons with Ken Jennings?"